A new method for determining surface concentrations of marine natural products on seaweeds
نویسندگان
چکیده
To understand the role of marine natural products in surface mediated ecological interactions-such as biofouling-we need to be able to quantify metabolites at or near the relevant surfaces We describe a new technique for determining the concentration of natural products on the surface of marine algae. Surface metabolltes were quantified for 2 red algae, Delisea pulchra and Laurencia obtusa, by dipplng the algae in hexane for 20 to 40 s at room temperature. This allowed for quantitative extraction of non-polar natural products from the surface of the thalli without disrupting cells (as determined by epifluorescence nucroscopy) More s t r~ngent extraction procedures using other solvents, or longer extraction times in hexane (>50 S ) , caused significant quantifiable cell damage. Natural products In the surface extracts were then measured uslng gas chromatography-mass spectroscopy (gc-ms). Mean total surface concentration of natural products from D. pulchra were 250 ng cm-" but < l ng cm-2 for L . obtusa. These results contrast to whole plant levels of total secondary metabolites in the 2 algae, w h ~ c h W C ~ C significantly higher in L. obtusa (7 pg mg-2 dry weight) than in D. pulchra (3.4 1-19 mg-2 dry weight) Dipping thalli in hexane for 30 S also caused no cell lysis in 8 other species of macroalgae This suggests that the procedure is more broadly applicable for the quantification of non-polar surface metabolites on seaweeds, and other organisms with resistant surface cells Our results h~ghl ight he need to deternune where compounds occur, and at what concentration, if we are to understand their ecological roles.
منابع مشابه
In vitro investigating of anticancer activity of focuxanthin from marine brown seaweed species
Breast cancer is the most common cancer type among women all over the world. Chemotherapy is the use of anticancer medicines for treating cancer but it has many side effects and cells may become resistant to these chemical medicines. Therefore, finding new compounds of natural origin could be a promising solution to this problem. The aim of the current study was to evaluate anticancer activity ...
متن کاملRole of seaweed (Ulva flexuosa Wulfen) extract in improvement of postharvest quality of Washington navel orange fruits
Seaweeds are rich sources of bioactive natural products, which have been considered as potential biotic agents. Ulva flexuosa Wulfen is a species of seaweed which is endemic to the Persian Gulf. In this study, the effect of different concentrations of U. flexuosa extract on the antioxidant activity of Washington Navel orange (Citrus sinensis L. cv. Washington Navel)...
متن کاملResearch Article: Extraction of abscisic acid and gibberellin from Sargassum muticum (Phaeophyceae) and Gracilaria corticata (Rhodophyta) harvested from Persian Gulf
Phytohormones are present in seaweeds but little is known about occurrence and content of them in seaweeds of Persian Gulf. The aim of this study was extraction of abscisic acid and gibberellin in Sargassum muticum and Gracilaria corticata. The seaweeds were collected bimonthly over one year at Bushehr coasts, Persian Gulf, during a range of environmental conditions. We explored new HPLC method...
متن کاملToxicity Assessment of SiO2 Nanoparticles to Pear Seedlings
To date, the effects of nanoparticles on woody plants remain unaddressed. This study reveals some of the physiological and biochemical effects of SiO2 nanoparticles on wild pear seedlings. The seedlings were irrigated with different concentrations of nano silica (0, 10, 100, 500 and 1000 mg/l) for 14 days. Nanoparticle adsorption and absorption, biomass allocation, gas exchange, relative water ...
متن کاملDesign and synthesis of marine natural product-based 1H-indole-2,3-dione scaffold as a new antifouling/antibacterial agent against fouling bacteria.
Marine organisms such as seaweeds, sponges and corals protect their own surfaces from fouling by their high anesthetic, repellant, and settlement inhibition properties. Within the marine ecosystem, evolution has allowed for the development of certain antifouling properties. Isatin is a biologically active chemical produced by an Alteromonas sp. strain inhibiting the surface of embryos of the ca...
متن کامل